The paint contains a “photo-luminising” powder that charges up in the daytime and slowly releases a green glow at night, doing away with the need for streetlights.
Interactive artist Daan Roosegaarde teamed up with Dutch civil engineering firm Heijmans to work on the idea.
The technology is being tested with an official launch due later this month.
It is the first time “glowing lines” technology has been piloted on the road and can be seen on the N329 in Oss, approximately 100km south east of Amsterdam.
Once the paint has absorbed daylight it can glow for up to eight hours in the dark.